sos1 exchange domain (Cytoskeleton Inc)

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Cytoskeleton Inc sos1 exchange domain
Sos1 Exchange Domain, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sos1+exchange+domain/SOS1+Exchange+Domain+Protein/us12552787-643-15-25
Average 94 stars, based on 1 article reviews

sos1 exchange domain - by Bioz Stars, 2026-10

94/100 stars

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Concentration Assay:

Article Title: Contribution of Noncovalent Recognition and Reactivity to the Optimization of Covalent Inhibitors: A Case Study on KRas G12C
Article Snippet: .. The nucleotide exchange reaction was initiated by adding a 100-fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a nonhydrolyzable GTP analog, and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton, Inc.) protein in 0.2 μM final concentration. .. The change in fluorescence intensity was measured every 30 s in RT for 60 min on an EnvSpire plate reader (PerkinElmer, Inc.).

Article Title: Contribution of Noncovalent Recognition and Reactivity to the Optimization of Covalent Inhibitors: A Case Study on KRas G12C .
Article Snippet: .. The nucleotide exchange reaction was initiated by adding a 100-fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a nonhydrolyzable GTP analog, and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton, Inc.) protein in 0.2 μM final concentration. .. The change in fluorescence intensity was measured every 30 s in RT for 60 min on an EnvSpire plate reader (PerkinElmer, Inc.).

Article Title: Size-Dependent Target Engagement of Covalent Probes
Article Snippet: .. The nucleotide exchange reaction was initiated by adding a 100-fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a nonhydrolyzable GTP analog, and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton, Inc.) protein at a final concentration of 0.2 μM. .. The change in fluorescence intensity was measured every 30 s in RT for 60 min on an EnSpire plate reader (PerkinElmer, Inc.).

Article Title: Mapping protein binding sites by photoreactive fragment pharmacophores
Article Snippet: .. The nucleotide exchange reaction was initiated by adding 100 fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a non-hydrolyzable GTP analog and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton, Inc.) protein in 0.5 μM final concentration. .. The change in fluorescence intensity was measured every 30 s in room temperature for 60 min on an EnSpire plate reader (PerkinElmer, Inc.).

Article Title: Size-Dependent Target Engagement of Covalent Probes.
Article Snippet: .. The nucleotide exchange reaction was initiated by adding a 100-fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a nonhydrolyzable GTP analog, and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton, Inc.) protein at a final concentration of 0.2 μM. .. The change in fluorescence intensity was measured every 30 s in RT for 60 min on an EnSpire plate reader (PerkinElmer, Inc.).

Article Title: Fragment‐Based Screening of Heterocyclic Electrophiles Identified Acrylamide Bioisoteres for Covalent KRas G12C Inhibitors
Article Snippet: First, the MANT‐GDP bound KRAS G12C protein (in a final 1 μM molar concentration) was incubated with the inhibitor molecules for 60 min. After the incubation, the MANT‐GDP KRAS‐inhibitor mixtures were loaded into a black 384 well microplate in the presence or absence of the inhibitor molecule in various concentrations. .. The nucleotide exchange reaction was initiated by adding 100 fold molar excess of GppNHp (catalog no.: G0635, Sigma‐Aldrich), a non‐hydrolysable GTP analog and the SOS1 exchange domain (catalog no.: GE02, Cytoskeleton Inc.) protein in 0.2 μM final concentration. .. The change in fluorescence intensity was measured every 45 s in room temperature for 90 min on an EnvSpire plate reader (PerkinElmer Inc.).

Purification:

Article Title: Quinazoline compound
Article Snippet: .. Experimental Materials: KRAS (G12C) protein was expressed and purified by AtaGenix Laboratories (Wuhan) Co., Ltd.; SOS1 exchange domain (564-1049) protein (Human recombinant) was purchased from Cytoskeleton; Mab Anti 6HIS-XL665 and Mab Anti GST-Eu cryptate were purchased from Cisbio. .. Multifunctional microplate reader Nivo5 was purchased from PerkinElmer.

Recombinant:

Fluorescence:

Article Title: Mapping protein binding sites by photoreactive fragment pharmacophores.
Article Snippet: .. The nucleotide exchange reaction was initiated by adding 100 fold molar excess of GppNHp (catalog no.: G0635, Sigma-Aldrich), a nonhydrolyzable GTP analog and the SOS1 exchange domain (catalog no.: GE02,Cytoskeleton, Inc.) protein in 0.5 μMfinal concentration.The change in fluorescence intensity was measured every 30 s in room temperature for 60min on an EnSpire plate reader (PerkinElmer, Inc.). .. The measured fluorescence values were fitted to a single exponential function by using GraphPad Prism 8 software.

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